20 L Bench-Scale Stirred Tank (Rushton)
Time: 0.00 h (Day 0.0)
+ Glucose Feed Pulse Injected
OD600: 0.82
Shear: 12.4 s⁻¹
Turbidity: Low (Clear)
Tap to rotate
Agitation:
450 RPM
Aeration:
1.20 vvm
Biomass X
g/L
0.80
Growth μ:
0.31 h⁻¹
Substrate S
g/L
35.00
Consumed:
0.0 %
Dissolved O₂
% SAT
100.0
kla:
65.0 h⁻¹
Target Product
g/L
0.00
Yield Y_P/S:
0.85
SCADA Telemetry Stream
60 FPS
Lag Phase
Biomass
Substrate
DO
Product
Process Disturbance Sandbox
NORMAL OPERATION
Auto DO:
Inject Disturbance:
Luedeking-Piret Kinetics:
Mixed
Growth-Assoc (α):
0.15 g_P/g_X
Non-Growth (β):
0.010 g_P/g_X/h
Safety Interlocks
Hypoxia Risk:
Safe (>20%)
Shear Stress:
Optimal (<3.0 m/s)
PID Actuator:
Manual Standby
Ashray et al. & Pioreactor continuous telemetry standard
HYDRODYNAMICS & SCALE
Reynolds (Re): 42,500
Tip Speed (v_tip): 2.12 m/s
Power P/V: 1.82 kW/m³
Flow Regime: Fully Turbulent
CARBON BALANCE AUDITOR
C-Carbon Fed: 14.0 g C/L
C-Incorporated: 5.8 g C/L
CO₂ Respiration: 8.2 g C/L
Recovery Balance: 99.8%
MEDIA COMPOSITION & C/N
Dissolved Nitrogen: 5.00 g/L
C/N Mass Ratio: 2.80 (Optimal)
Culture Purity: 100.0%
Osmolarity: 290 mOsm
THERMAL & pH CONTROLLERS
Broth Temp:
30.0 °C
Broth pH:
6.85 pH